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A potent, bio‐degradable eco‐friendly organic waste degrader for exceptional odour control in portable toilet, entertainment, air liner, mining, recreational vehicle, and marine holding tank applications.

Current Challenges:

The current generally accepted ways of treating portable toilet effluent balance a number of complex challenges, with serious compromises on each front ranging from under performance, health risks to humans, to enormously damaging consequences to the environment.

Formaldehyde & Gluteraldehyde: These are known cancer causing carcinogens and are definitely not ideal where any kind of exposure to humans is likely. Not readily bio‐ degrade and extremely damaging to the environment when they are discharged, they cause numerous problems when ending up at waste water sewage treatment plants.

Enzyme & Bacterial Solutions: Much safer for humans, they are actually beneficial to the environment when discharged. They have their own serious and limiting applications however.

Enzymes are subject to a narrow effective temperature range. Just as humans run the fatal risk of denaturing enzymes at fevers of 40C or very low temperatures, portable toilet applications face sweltering outdoor temperatures especially when adding the greenhouse warming effects one experiences like in a closed motor vehicle on a hot sunny day, temperatures quickly pass the point where enzymes die. In open environments the same applies to very cold environments where enzymes can quickly die due to the cold. Importantly enzymes are very target specific, and if the easy mistake is made of using the wrong enzymes in formulation, the solution will be ineffective at odour control. Crucially enzymes are not able to reproduce, their numbers are limited to the initial dose amount introduced.

Bacteria are much hardier than enzymes. Think of how one needs to boil water first to eliminate bacteria, they are more resistant to temperature changes. Compared to enzymes bacteria have the added benefit of being able to reproduce at exponential rates. Bacteria then produce the right combination of enzymes to effectively break down the waste on hand. But where enzyme solutions start working immediately, bacterial solutions take a long period to become effective and are only suitable for low traffic environments. Most notable with both enzymes and bacteria, as soon as the very real scenario of chemicals being introduced comes to play, both solutions will die off and effectively become useless. Both solutions also have logistically challenging very limited shelf lives.

The Solution:

Ecozyme Porta-Loo is a proprietary high performance formulation which is impervious to temperature changes, works immediately and on an ongoing basis, is bio‐degradable and suitable for wastewater plants, is non‐carcinogenic and safe for humans, is non‐volatile, does not have a limited shelf life, and continues to work even when chemicals are invariably dumped into the portable toilet environment. Crucial to portable toilet environments is maintaining a suitable and constant PH situation. Ecozyme Porta-Loo achieves this with a complex proprietary PH buffer system. Odours are quickly removed and further masked with a powerful fragrance system.

Product Features:

‐High odour control properties

‐Biodegradable and eco friendly

‐Wide temperatures operation

‐Non‐volatile and non‐carcinogenic

‐Suitable for wastewater plants

‐Does not get killed by chemicals

Intelligent PH buffer system

Case Study:

Chemical Toilets: Deodorant concentrates were prepared based on nonionic surfactant, fragrance, buffer, dye and water.

Biocides were added at appropriate levels to provide two formulations, one containing a Ecozyme Porta-Loo formulated product and the other Formaldehyde ‐ a common toilet fluid biocide.

These products were then diluted to achieve in‐use concentrations of 200ppm (Ecozyme Porta-Loo) and 1875ppm (Formaldehyde) respectively.

The dilutions were challenged initially with Escherichia coli at 109 colony forming units (cfu)/ml. Thereafter, daily challenges were continued at 105 cfu/ml. At each challenge, tryptic soy broth was added as a simulated organic loading.

All samples were stored at 37 ̊C throughout the 2‐week test. At suitable intervals, samples were removed and assessed using standard plate count methods.

The results are presented in Figure 1 on the following column:

Figure 1 ‐ Efficacy of Ecozyme Porta-Loo in a simulated chemical toilet fluid

Safety Characteristics:

A considerable data package has been assembled on Ecozyme Porta-Loo, to support a wide rang

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